Table 1.
RT-PCR primers used in this study
| Details of primers used for RT-PCR | |||
|---|---|---|---|
| Target transcript | Accession number | Oligonucleotide sequences (5′-3′) (+): sense (−): antisense | Fragment length (bp) |
| Gapdh | NM_008084 | GTCTTCACCACCATGGAGA (+) | 190 |
| AAGCAGTTGGTGGTGCAG (−) | |||
| Kcnj8 (Kir6.1) | NM_008428 | CTCTTCCCTCATGGTGCCCAAGGTG (+) | 190 |
| CGGAACTCAATGAACTGTCTCCGGA (−) | |||
| Kcnj11 (Kir6.2) | NM_010602 | TCTGCTTTCAGCTCCCATGGGCAG (+) | 200 |
| TGGAGTTCTGGGCAAGGACACCACA (−) | |||
| Abcc9 (SUR2A) | NM_021041 | CCAGCGTGATCTGCTCCGACAAGTG (+) | 167 |
| CTGCACGGACAAACGAGGCAAACAC (−) | |||
| Abcc9 (SUR2B) | NM_021041 | AGTCATGACAGCCTTTGCGGATCGC (+) | 172 |
| TGCACGGACAAACGAGGCAAACACT (−) | |||
All primers were designed against Mus musculus GenBank sequences. Gapdh (glyceraldehyde-3-phosphate dehydrogenase).