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. 2011 Jun;163(4):815–825. doi: 10.1111/j.1476-5381.2011.01263.x

Figure 2.

Figure 2

20-Hydroxyeicosatetraenoic acid (20-HETE) attenuated ATPγS-induced COX-2 expression in vascular smooth muscle cell. Quiescent vascular smooth muscle cell were pre-incubated with 20-HETE (5 or 10 µM) or TGF-β (200 pg·mL−1) for 20 h (A,C) or 22 h (B) before incubation with ATPγS (100 µM) for 2 h (B) or 4 h (A,C). Total protein and RNA was subjected to analysis of COX-2 expression by Western blot (A, n = 6–11; C, n = 3) and quantitative-PCR (B, n = 3), respectively. Values are mean ± SE. *P < 0.05 compared with corresponding control groups. #P < 0.05 compared with corresponding group without 20-HETE.