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. 2011 Jun;163(4):815–825. doi: 10.1111/j.1476-5381.2011.01263.x

Figure 5.

Figure 5

20-Hydroxyeicosatetraenoic acid (20-HETE) inhibited activated protein-1 (AP-1) mediated transcription of COX-2 via peroxisome proliferator activator receptor-α (PPARα). The cells were transiently transfected with the COX-2 promoter luciferase plasmid containing AP-1 binding site for 24 h, followed by pre-incubation with 20-HETE for 20 h before incubation with ATPγS (100 µM; A). After transfection, quiescent vascular smooth muscle cell were pre-incubated with MK886 (2 µM) in the presence or absence of 20-HETE (10 µM; B) or WY14643 (100 µM; D) for 20 h before incubation with ATPγS (100 µM) and incubated for additional 4 h. The PPARα deficient or control cells were transfected with the COX-2 promoter luciferase plasmid containing AP-1 binding site for 24 h before incubation with ATPγS in the presence or absence of 20-HETE (C) or WY14643 (E). Values are presented as mean ± SEM. *P < 0.05 compared with corresponding control groups. #P < 0.05 compared with corresponding group without 20-HETE. †P < 0.05 compared with the indicated group.