Multiple sequence alignment between human CRBP III and previously
characterized CRBPs. Residues that are identical or similar in at least
five of the six sequences are boxed in black or gray, respectively. The
symbols below amino acid letter residues denote: ○, residues
that in rat retinol–CRBP I change their accessible surface area by
more than 1 Å2 after ligand removal (4); +, residues that
have an atom within a distance of 3.6–5.1 Å from bound retinol in rat
retinol–CRBP II (5); ×, residues that have an atom within 3.6 Å of
bound retinol in rat retinol–CRBP II (5). The positions of β-strands
A-J (arrows) and α-helices A and B (boxes) are indicated according to
ref. 4. Conservative substitutions are defined as follows:
A/I/L/M/V, S/T, F/Y/W, D/E, N/Q, and K/R. GenBank
accession nos. are: P09455 and P50120, human (h) CRBP I and II,
respectively; P02696 and P06768, rat (r) CRBP I and II, respectively;
CA885608, gecko (g) (Lygodactylus picturatus)
ι-crystallin/CRBP.