Fig. 2.

Rat and mouse NKR-P1F and NKR-P1G reporters show overlapping specificities against a panel of tumour target cells. a Expression of FLAG-tagged chimeric molecules was detected by staining with the anti-FLAG mAb, M2 (shaded histograms). Open histograms show control staining with only secondary antibody. Reporter cells (r rat and m mouse) were co-incubated overnight with rat b or mouse c tumour cell lines. Ligand-induced stimulation of chimeric receptors was measured as an EGFP signal by flow cytometry. Numbers represent percentage and MFI values (in parenthesis) of positive cells. One out of three representative experiments is shown