Fig.3.

Effects of increasing concentrations of OEA (A) and KDS-5104-5104 (B) on [³H]anandamide (AEA) hydrolysis by recombinant FAAH following no preincubation or 2 h or preincubation of the enzyme with OEA or KDS-5104, respectively. The IC₅₀ for OEA at 0 h was 5.46 ± 0.3 µM and 15.0 ± 1.5 µM at 2 h; the IC₅₀ for KDS-5104 at 0 h was 92.2 ± 4.6 µM and 98.8 ± 11.6 µM at 2 h. Hydrolysis of OEA (C) and KDS-5104-5104 (D) is shown, as measured by HPLC-MS after 0 h or 2 h of incubation with brain or liver mice homogenate.