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. 2010 Dec;31(36):9395–9405. doi: 10.1016/j.biomaterials.2010.08.051

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© 2010 Elsevier Ltd.

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Fig. 3 — Expression of KSI-rP₁₁-4 fusion protein. SDS-PAGE analysis of KSI-rP₁₁-4 expressed in E. coli BL21 (DE3) Star cells by auto-induction from 18 to 72 h in (A) soluble and (B) insoluble fractions. U_N, uninduced control in which no lactose was added to the culture medium and grown for 72 h. (C) Changes in culture densities () and protein yields () over time. Data expressed as (n = 3) ± 95% confidence limits. TEM analysis of E. coli cells carrying a pET31b-KSI-rP₁₁-4 construct (D) induced showing dense inclusion body formation and (E) uninduced showing no inclusion body formation.

Inline graphic — Expression of KSI-rP₁₁-4 fusion protein. SDS-PAGE analysis of KSI-rP₁₁-4 expressed in E. coli BL21 (DE3) Star cells by auto-induction from 18 to 72 h in (A) soluble and (B) insoluble fractions. U_N, uninduced control in which no lactose was added to the culture medium and grown for 72 h. (C) Changes in culture densities () and protein yields () over time. Data expressed as (n = 3) ± 95% confidence limits. TEM analysis of E. coli cells carrying a pET31b-KSI-rP₁₁-4 construct (D) induced showing dense inclusion body formation and (E) uninduced showing no inclusion body formation.