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Chromosome Abnormalities
Peripheral blood mononuclear cells were isolated from venous blood samples as described in Methods. After phytohaemagglutinin stimulation, DNA damage was induced by 2Gy radiation therapy and DNA repair was inhibited by incubation with 100μM hydroxycarbamide. After lymphocytes were harvested and stained, 100 metaphase cells were examined for evidence of DNA damage, including chromatid breaks (A), chromosome breaks (B), and chromosome fusion events (C).
