Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Expert Rev Mol Diagn. 2011 Mar;11(2):159–169. doi: 10.1586/erm.10.115

Figure 6. High-resolution melt analysis of TP53 exon 6 amplification products produced via conventional and coamplification at lower denaturation temperature (COLD)-PCR.

Figure 6

The amplicons were produced from genomic DNA serial dilutions of the human cell line SNU-182 (c.644G>T, p.S215I) in WT DNA. While the 2% mutant abundance is the lower limit of detection in the conventional PCR amplicons, COLD-PCR enriches the mutant fraction so that an initial mutant abundance as low as 0.1% can now be detected.

WT: Wild-type.

Data taken from [37].