Abstract
Upon base composition analysis, oligonucleotides which are labeled at the 3'-terminus with fluorescein or biotin generate an additional, late eluting peak in the HPLC chromatogram. Investigation of this effect revealed the haptens acted as apurinic sites, and phosphodiesterase cleavage of the phosphate bond between the upstream nucleotide and apurinic site is inhibited. Extension of this work with a base-stable apurinic site inserted into all possible junctures of 5'-TGAC-3' tetramers showed this to be a general effect. As a consequence of this work, acid-catalyzed depurination resulting in apurinic sites can be monitored in oligonucleotide synthesis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barone A. D., Tang J. Y., Caruthers M. H. In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports. Nucleic Acids Res. 1984 May 25;12(10):4051–4061. doi: 10.1093/nar/12.10.4051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gaffney B. L., Marky L. A., Jones R. A. Synthesis and characterization of a set of four dodecadeoxyribonucleoside undecaphosphates containing O6-methylguanine opposite adenine, cytosine, guanine, and thymine. Biochemistry. 1984 Nov 20;23(24):5686–5691. doi: 10.1021/bi00319a004. [DOI] [PubMed] [Google Scholar]
- Luebke K. J., Dervan P. B. Nonenymatic ligation of double-helical DNA by alternate-strand triple helix formation. Nucleic Acids Res. 1992 Jun 25;20(12):3005–3009. doi: 10.1093/nar/20.12.3005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suematsu E., Resnick M., Morgan K. G. Ca(2+)-independent change in phosphorylation of the myosin light chain during relaxation of ferret aorta by vasodilators. J Physiol. 1991;440:85–93. doi: 10.1113/jphysiol.1991.sp018697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinfeld M., Liuzzi M., Paterson M. C. Selective hydrolysis by exo- and endonucleases of phosphodiester bonds adjacent to an apurinic site. Nucleic Acids Res. 1989 May 25;17(10):3735–3745. doi: 10.1093/nar/17.10.3735. [DOI] [PMC free article] [PubMed] [Google Scholar]