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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Cancer Invest. 2011 Jul;29(6):383–395. doi: 10.3109/07357907.2011.584588

Fig. 9. The H11/HspB8 mutant P173H does not bind TAK1.

Fig. 9

(A) Reciprocal pull-down assay of protein extracts from MeWo cells (express P173H) using antibodies to TAK1 (T), H11/HspB8 (H), or normal IgG (Ig) in immunoprecipitation (IP) and immunoblotting (IB). (B) Reciprocal pull-down assay of protein extracts from SKMEL-2 cells stably transfected with tet-regulated H11/HspB8 untreated or treated with Dox (5μg/ml; 3d) done as in (A). Molecular weights are shown on the right. * indicates slower migrating phosphorylated TAK1 protein.