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. Author manuscript; available in PMC: 2011 Dec 2.
Published in final edited form as: Nat Protoc. 2011 Jun 2;6(6):870–895. doi: 10.1038/nprot.2011.328

Figure 1.

Figure 1

A typical cell cycle profile for a mammalian fibroblast population obtained during FACS analysis by plotting cell count vs. DNA content. In this example, cellular DNA was stained with PI, so DNA content is represented by PI intensity. A G1 peak, representing cells with 2N DNA content, and a G2/M peak, representing cells that have undergone replication and therefore possess a 4N DNA content are labeled. The area between these two peaks is representative of cells in S phase, and can be sorted into two fractions, as indicated here, to obtain early and late S phase samples.