| Component | Amount per reaction (μL) | Final |
|---|---|---|
| 10X Taq buffer | 1.25 | 1X |
| 10mM dNTPs | 0.25 | 0.2 mM |
| 20 U/μL Taq Polymerase | 0.06 | 1.2 U |
| F/R 20 μM combined primers | 0.31 | 0.5 μM |
| Nuclease free water | to 12.5 |
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