Table 1.
Proteins identified by liquid chromatography-tandem mass spectrometry
| Description | MW (kDa) |
Found in CVF (PW) |
Found in CVF (HW) |
Found in CM |
Found in amnion |
Found in AF |
|---|---|---|---|---|---|---|
| 21 kDa protein | 20.8 | d | ||||
| Actin, cytoplasmic 1 | 41.7 | d | f | h | h | |
| Actinin, alpha 1 isoform c | 102.6 | |||||
| Afamin | 69.0 | d | f | i,j | ||
| Alpha-1-acid glycoprotein 1 | 23.5 | a,c | d | g | h,j | |
| Alpha-1-antichymotrypsin | 47.6 | a,b | d | f,g | i | |
| Alpha-1-antitrypsin | 46.7 | a,b,c | d | f,g | h,i | |
| Alpha-1B-glycoprotein | 54.2 | f | h,i,j | |||
| Alpha-2-macroglobulin | 163.2 | d | f | i | ||
| Alpha-2-macroglobulin-like protein 1 | 161.0 | |||||
| Alpha-enolase | 47.1 | e | g | h | ||
| Aminopeptidase N | 109.5 | h | ||||
| Angiotensinogen | 53.1 | f | i,j | |||
| Annexin A1 | 38.7 | a,b | d,e | f,g | h | |
| Annexin A2 | 38.6 | a,b | d,e | f,g | h | |
| Annexin A3 | 36.4 | a,c | d,e | f,g | h | |
| Annexin A5 | 35.8 | c | e | f,g | h | |
| Antileukoproteinase | 14.3 | a | d | g | j | |
| Antithrombin-III | 52.6 | d | f | i,j | ||
| Apolipoprotein A-I | 30.8 | a | e | f,g | h | h,i,j |
| Apolipoprotein A-II | 11.2 | i,j | ||||
| Apolipoprotein A-IV | 45.4 | f | h,i | |||
| Apolipoprotein B-100 | 515.2 | i | ||||
| Azurocidin | 26.9 | d | f | |||
| Carbonic anhydrase 1 | 28.9 | f,g | h | |||
| Catalase | 59.7 | a | d,e | f | ||
| Cathelicidin antimicrobial peptide precursor | 19.6 | g | ||||
| Cathepsin G | 28.8 | a,b | d | f | ||
| CD81 antigen | 25.8 | |||||
| Ceruloplasmin | 122.1 | a | d,e | f,g | i,j | |
| Chloride intracellular channel protein 1 | 26.9 | e | f | h | ||
| Chondroadherin | 40.5 | |||||
| Clusterin | 52.3 | d | f | i,j | ||
| Cofilin-1 | 18.5 | f | h | |||
| Collagen alpha-2(I) chain | 129.2 | d | j | |||
| Complement C3 | 187.0 | a | d,e | f,g | i,j | |
| Complement C4-A | 192.7 | a | g | i,j | ||
| Complement C5 | 188.2 | |||||
| Complement component 4B preproprotein | 192.6 | a | d | f | i,j | |
| Complement component 6 precursor | 105.7 | d | f | |||
| Complement component C8 gamma chain | 22.3 | |||||
| Complement factor B | 85.5 | d | g | h,i,j | ||
| Complement factor H | 139.0 | d | f | j | ||
| Cystatin-A | 11.0 | a,c | d,e | f,g | j | |
| Cysteine-rich secretory protein 3 | 27.6 | a | d | f | ||
| Cytidine deaminase | 16.2 | d | ||||
| DKFZp686C11235 | 52.1 | |||||
| DKFZp686C15213 | 51.1 | |||||
| DKFZp686D0114 | 30.5 | |||||
| DKFZp686I04196 | 46.0 | |||||
| DKFZp686M24218 | 52.4 | |||||
| Fatty acid-binding protein, epidermal | 15.2 | a,b,c | d,e | f,g | h | |
| Ferritin | 11.2 | f | ||||
| Fibronectin | 259.0 | c | d | f | i,j | |
| Fibulin-1 | 74.4 | d | f | j | ||
| Galectin-3-binding protein | 65.3 | d | f | |||
| Glutaredoxin-1 | 11.8 | d | ||||
| Glyceraldehyde 3-phosphate dehydrogenase | 28.6 | a,b,c | d,e | f,g | ||
| Haptoglobin | 46.7 | a,c | d,e | f,g | h | |
| Hemoglobin subunit alpha | 15.2 | b | d | f,g | h | h |
| Hemoglobin subunit beta | 16.0 | d | g | |||
| Hemoglobin subunit delta | 16.0 | d | f | |||
| Hemopexin | 51.6 | a | d | f,g | i,j | |
| Histone H2A type 2-C | 14.0 | a | ||||
| Histone H4 | 11.4 | a,b,c | d | g | ||
| Ig gamma-3 chain C region | 41.3 | j | ||||
| Ig kappa chain C region | 25.4 | a,b | d | g | h | |
| Ig kappa chain V-I region OU | 11.8 | g | ||||
| Ig kappa chain V-II region TEW | 12.3 | a | g | |||
| Ig kappa chain V-III region B6 | 11.6 | g | ||||
| Ig kappa chain V-IV region Len | 12.6 | a | d | g | ||
| Ig mu chain C region | 49.3 | a,b,c | ||||
| IGFBP1 | 22.9 | h,i,j | ||||
| IgGFc-binding protein | 571.6 | d | g | |||
| Immunoglobulin J chain | 18.1 | a | d | f | ||
| Inter-alpha (globulin) inhibitor H2 | 105.2 | i | ||||
| Inter-alpha (globulin) inhibitor H4 isoform 2 | 99.8 | f | i | |||
| Inter-alpha-trypsin inhibitor heavy chain H1 | 101.3 | |||||
| Keratin 13 isoform b | 45.8 | f | ||||
| Keratin, type I cytoskeletal 10 | 58.8 | f | ||||
| Keratin, type I cytoskeletal 14 | 51.5 | |||||
| Keratin, type I cytoskeletal 16 | 51.2 | f | ||||
| Keratin, type I cytoskeletal 9 | 62.0 | f | i | |||
| Keratin, type II cytoskeletal 1 | 66.0 | b | f | h | h,i | |
| Keratin, type II cytoskeletal 2 epidermal | 65.8 | f | ||||
| Keratin, type II cytoskeletal 5 | 62.3 | f | ||||
| Leukocyte elastase inhibitor | 42.7 | a,b | d | h | ||
| Long palate, lung and nasal epithelium carcinoma-associated protein 1 | 52.4 | a | ||||
| Lumican | 38.4 | i,j | ||||
| Lysozyme C | 16.5 | a,b,c | d,e | f,g | j | |
| Mammaglobin-B | 10.9 | |||||
| Matrix metalloproteinase-9 (MMP-9) | 78.4 | a | d | f | ||
| MUC1 isoform T9 | 12.1 | |||||
| Mucin-16 | 2351.2 | d | ||||
| Mucin-5AC | 526.3 | |||||
| Mucin-5B | 590.4 | a,c | d | j | ||
| Myeloblastin | 27.8 | a | d | g | ||
| Myeloperoxidase | 73.8 | a,b | d | f | ||
| Myosin-reactive immunoglobulin heavy chain variable region | 13.2 | d | i | |||
| Myosin-reactive immunoglobulin light chain variable region | 11.5 | d | h | |||
| Neutrophil defensin 1 | 10.2 | a | d | g | ||
| Neutrophil elastase | 28.5 | |||||
| Neutrophil gelatinase-associated lipocalin | 22.6 | a,b,c | d,e | g | j | |
| Peptidoglycan recognition protein | 21.7 | |||||
| Pigment epithelium-derived factor | 46.3 | i | ||||
| Plasma protease C1 inhibitor | 55.1 | d | ||||
| Plasma serine protease inhibitor | 45.7 | d | f | i | ||
| Plasminogen | 90.5 | a | d | i,j | ||
| Plastin-2 | 70.2 | d | g | |||
| Polymeric immunoglobulin receptor | 83.2 | a,b | d | f | i,j | |
| Pregnancy zone protein | 163.8 | |||||
| Profilin-1 | 15.0 | a,c | d,e | f,g | h | h |
| Progestagen-associated endometrial protein (Glycodelin-A) | 14.9 | j | ||||
| Prolactin-inducible protein | 16.6 | d | ||||
| Prominin 1 isoform 4 | 92.7 | f | ||||
| Protein AMBP | 39.0 | j | ||||
| Protein S100-A11 | 11.7 | d | f,g | |||
| Protein S100-A8 | 10.8 | a,b,c | d | f,g | h | |
| Protein S100-A9 | 13.2 | a,b,c | d | f,g | h,i | |
| Resistin | 11.4 | d | ||||
| Retinol binding protein 4, plasma | 22.9 | |||||
| Rheumatoid factor D5 light chain | 12.8 | |||||
| Ribonuclease T2 | 29.5 | d | ||||
| Secretoglobin family 1D member 2 | 9.9 | |||||
| Serotransferrin | 77.0 | a,c | d | h | h,j | |
| Serpin B3 | 44.5 | d,e | g | |||
| Serum albumin | 69.3 | a | e | g | h,i,j | |
| Serum paraoxonase/arylesterase 1 | 39.7 | f | ||||
| Sulfhydryl oxidase 1 | 66.8 | d | ||||
| Thioredoxin, isoform CRA_b | 9.4 | a | e | h | j | |
| TIMP metallopeptidase inhibitor 1 | 16.0 | |||||
| Transaldolase | 37.5 | a | d | g | ||
| Transcobalamin-1 | 48.2 | d | ||||
| Transthyretin | 15.9 | d | f,g | i,j | ||
| Vitamin D-binding protein | 52.9 | a,c | d,e | h,i,j | ||
| Vitronectin | 54.3 | a | f,g | i,j | ||
| Zymogen granule protein 16 homolog B | 22.7 |
Identified proteins in the list were found in at least 2 of 5 CMPs. These proteins were also found in other studies of CVF, amnion, or AF:
a
ref (27);
b
ref (28);
c
ref (29);
d
ref (30);
e
ref (31);
f
ref (16);
g
ref (17);
h
ref (32);
i
ref (33);
j
ref (34).
MW: theoretical molecular weight, CMP: cervical mucus plug, CVF(PW): cervicovaginal fluid of pregnant women, CVF(HW): cervicovaginal fluid of non-pregnant women, CM: cervical mucus, AF: amniotic fluid