Table 1.
Results of 16S PCR testing of canine pRBC units inoculated with 5 or 25 μL of the Pseudomonas fluorescens (Pf)–contaminated sentinel unit and stored at 4°C and 20°C for up to 72 hours.
| Storage Temperature | Inoculum (μL of Pf‐rich pRBCs) | 16S PCR Results | ||||||
|---|---|---|---|---|---|---|---|---|
| Incubation Period (Hours) | ||||||||
| 0 | 4 | 8 | 24 | 48 | 52 | 72 | ||
| 4°C (moved to 20°C after 48 hours) | 0 | − | − | − | − | − | ND | − |
| 0 (1 mL saline) | − | − | − | + (c−) | − | ND | − | |
| 5 | − | − | − | − | − | + | + | |
| 25 | − | − | − | − (c+) | + | + | + | |
| 20°C | 0 | − | − | − | − | − | ND | − |
| 0 (1 mL saline) | − | − | − | − | − | ND | − | |
| 5 | − | − | − | − | + | ND | + | |
| 25 | − | − | + | + (c+) | + | ND | + | |
Results for samples that were cultured are indicated as (c+), culture‐positive for P. fluorescens and (c−), culture negative. Shaded results indicate a color change was also observed.
+, positive; −, negative; ND, not done.