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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 13;51(1):160–170. doi: 10.1016/j.freeradbiomed.2011.04.007

Figure 1.

Figure 1

Room temperature EPR spectra of the superoxide radical adduct of DMPO, DMPO-OOH. All the reactions were performed in 50 mM phosphate buffer (pH = 7.4) containing 0.1 mM DTPA. Spectrum A: DMPO (50 mM), methyl-β-cyclodextrin (0.1 M), Fe3+cyt c (0.1 mM), NADH (1 mM), and H2O2 (0.5 mM). Spectrum B: DMPO (50 mM), methyl-β-cyclodextrin (0.1 M), NADH (1 mM), and H2O2 (0.5 mM). Spectrum C: DMPO (50 mM), Fe3+cyt c (0.1 mM), NADH (1 mM), and H2O2 (0.5 mM). Spectrum D: DMPO (50 mM), methyl-β-cyclodextrin (0.1 M), Fe3+cyt c (0.1 mM), SOD1 (500 U/mL), NADH (1 mM), and H2O2 (0.5 mM). Spectrum E: DMPO (50 mM), methyl-β-cyclodextrin (0.1 M), Fe3+cyt c (0.1 mM), and H2O2 (0.5 mM). Spectrum F: DMPO (50 mM), methyl-β-cyclodextrin (0.1 M), Fe3+cyt c (0.1 mM), and NADH (1 mM). The observed isotropic hyperfine values of the DMPO-OOH adducts are aN = 13.49 G, aH1 = 10.78 G, and aH2 = 1.39 G. EPR instrument parameters used were as described in the materials and methods section.