Figure 10.

The effect of NADH and H₂O₂ on the reduction of Fe³⁺cyt c in phosphate buffer, pH 7.4. Experiments were performed with Fe³⁺cyt c, NADH, and H₂O₂. Reactions were initiated by the addition of H₂O₂. UV-visible absorption spectra were recorded five minutes after mixing all the reactants. A: Fe³⁺cyt c (0.1 mM), NADH (0.1 – 2 mM), and H₂O₂ (0.5 mM). B: Fe³⁺cyt c (0.1 mM), NADH (1 mM), and H₂O₂ (0.05 – 0.5 mM). Data represent means ± S.E (n = 3).