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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 13;51(1):160–170. doi: 10.1016/j.freeradbiomed.2011.04.007

Figure 6.

Figure 6

Room temperature EPR spectra of the superoxide radical adduct of DMPO, DMPO-OOH. All the reactions were performed in 50 mM phosphate buffer (pH = 7.4) containing 0.1 mM DTPA. The reaction mixture contains DMPO (50 mM), methyl-β-cyclodextrin (0.1 M), Fe3+cyt c (0.1 mM), NADH (1 mM), and H2O2 (0.5 mM). Spectrum A: The reaction mixture was prepared inside the nitrogen filled glove box. Spectrum B: The reactants were taken out from the glove box and the reaction mixture was prepared under room air. EPR instrument parameters used were as described in the materials and methods section.