Figure 9.

UV-visible absorption spectra of cyt c in phosphate buffer, pH 7.4. Experiments were performed with Fe³⁺cyt c, NADH, and H₂O₂. Reactions were initiated by the addition of H₂O₂. Spectra were recorded five minutes after mixing all the reactants. A: Fe³⁺cyt c (0.1 mM), B: Fe³⁺cyt c (0.1 mM) and H₂O₂ (0.5 mM), C: Fe³⁺cyt c (0.1 mM) and NADH (2 mM), D: Fe³⁺cyt c (0.1 mM), NADH (2 mM), and H₂O₂ (0.5 mM).