Skip to main content
. 2011 Apr 11;66(7):1518–1524. doi: 10.1093/jac/dkr150

Figure 1.

Figure 1.

Effect of topA deletion on induction of recA::luxCDABE and dinD1::luxCDABE after mitomycin C treatment. E. coli RFM445 (topA+) and RFM475 (ΔtopA) transformed with either pDEW237 with the recA::luxCDABE fusion or pDinlux with the dinD1::luxCDABE fusion were grown to exponential phase (OD600 = 0.4) before the addition of mitomycin C. (a) Comparison of time course of luciferase response to 0.3 μM mitomycin C in RFM445 and RFM475. (b) Luciferase response ratio measured at 180 min after treatment with different concentrations of mitomycin C. The averages and standard deviations from three experiments are shown. MMC, mitomycin C.