Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Response of P19 EC cells to BMP4. (A) P19 EC cells are cultured in the presence or absence of BMP4 (0.5 μg/mL) and NOGGIN (1 μg/mL). (B) P19 EC cells are cultured in the presence or absence of BMP4 and SFRP1 (2.5 μg/mL). In both experiments, cells are cultured for 24 h and examined for the expression levels of Wnt3 and Brachyury by qRT-PCR. EC, embryonal carcinoma; qRT-PCR, quantitative reverse transcription–polymerase chain reaction.
