Table 1.

Relative Expression Levels of Cell-Autonomous Negative Regulators of Wnt/β-Catenin Signaling in Oct4-Knockdown P19 Cells

			Relative expression level (Oct4 shRNA/control shRNA)
Gene symbol	Reference	Agilent probe name	Microarray (Exp. 1–2)	qRT-PCRa(mean ± SD)
Gapdh	N/A	A_52_P1687	0.97–0.94	N/A
		A_52_P479958	0.96–0.96
Eef1a1	N/A	A_52_P299505	0.87–1.04	0.97 ± 0.21
(EF1α)		A_51_P118765	0.99–1.03
Actb	N/A	A_51_P173760	0.98–0.92	1.27 ± 0.16
(β-actin)		A_51_P251154	1.16–1.17
Gsk3a	[68]	A_51_P437279	1.05–1.23	0.97 ± 0.11
Gsk3b	[68]	A_52_P641629	1.37–1.66	1.42 ± 0.16
		A_51_P514412	1.14–1.97
Axin1	[69]	A_52_P197007	1.21–1.36	1.37 ± 0.35
Axin2	[69]	A_52_P54176	1.10–1.18	0.80 ± 0.04
Apc	[70]	A_52_P633752	1.22–1.20	1.33 ± 0.29
		A_51_P273378	1.89–2.07
		A_52_P284981	1.54–1.80
		A_52_P675171	1.37–1.76
		A_51_P469822	1.29–1.69
Apc2	[70]	A_51_P156708	0.99–0.97	0.42 ± 0.03
Btrc	[71]	A_52_P98674	1.09–1.08	1.15 ± 0.11
Ctnnbip1	[72]	A_51_P335849	1.27–1.45	1.51 ± 0.29
Cby1	[73]	A_51_P470311	0.80–0.98	0.70 ± 0.10
Tax1bp3	[74]	A_51_P487384	1.14–1.29	2.34 ± 0.08
Tle1	[75]	A_52_P328044	0.63–0.62	0.58 ± 0.09
		A_51_P199716	0.68–0.80
		A_52_P330694	0.79–0.98
Tle2	[75]	A_51_P175303	2.87–1.67	2.40 ± 0.86
Tle3	[75]	A_52_P590740	0.78–0.88	0.88 ± 0.08
		A_51_P151003	1.11–0.95
Tle4	[75]	A_51_P346543	2.21–2.43	2.30 ± 0.39
Tle6	[75]	A_51_P368591	0.74–0.69	0.44 ± 0.17
Aes	[75]	A_51_P243342	1.39–1.44	7.50 ± 1.88

^aNormalized with Gapdh.

qRT-PCR, quantitative reverse transcription–polymerase chain reaction.