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. 2011 May 5;11:75. doi: 10.1186/1471-2229-11-75

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Copyright ©2011 Aloui et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Impact of cadmium (Cd) and/or G. irregulare inoculation (Gi) on 2-DE shoot proteins profiles of M. truncatula relative to those non-treated; 600 μg phenol-extracted proteins were separated onto first-dimensional pH 3-10 non-linear IPG strips and second dimensional 12% vertical slab gels. Mw, Bio-Rad 2-DE molecular mass standards. Representative gels from Cd-treated plants (A), G. irregulare-inoculated plants (B), G. intraradices-inoculated Cd-treated plants (C) and non-treated control plants (D). Up- and down-headed arrows represent significant (n = 3, p < 0.05) increased and decreased protein abundance reproducibly quantified in two independent biological experiments compared to non-treated control plants, respectively.