Figure 5.

Validation of microarray data by in-situ hybridization. (a) In-situ localization of transcripts corresponding to the genes LOC_Os04g52550 and LOC_Os01g70440 in rice florets (MA, SCP and TPA stages as marked). Corresponding microarray-based expression profiles of these two genes are also shown as bar graphs for comparison. W, wall layers; V, vascular tissue; T, tapetum; M, microspores; MEI, meiocytes; L, lemma. (b) A compilation of in-situ localization analyses for six genes using published literature and their correlation with anther preferential expression profiles as revealed by the microarray analysis described in this paper. The log₂ normalized expression values were used to represent the gene specific microarray profiles.