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. 2001 Mar 27;98(7):3732–3737. doi: 10.1073/pnas.061467898

Figure 1.

Figure 1

Rapid and efficient reconstitution of TAP into proteoliposomes. Raji microsomes (6 mg/ml) were solubilized by DM (ρ = 2) and loaded on a Superose 6 column (A). Each fraction (60 μl) was analyzed by SDS/PAGE followed by immunoblotting by using specific monoclonal antibodies against TAP1 (mAb 148.3) (27), TAP2 (mAb 435.3) (9), MHC class I heavy chain (hc) (mAb A1.4) (38), and tapasin (mAb, R.T., unpublished work) (B). To study the efficiency of TAP reconstitution, equal aliquots of microsomal protein, after solubilization, isolation (fractions 19 to 21), and reconstitution into proteoliposomes, were analyzed by immunoblotting (C).