Table 1. Thermotolerant genes identified in this study.

Classification	Sub-classification	Gene	Function	Glca	Mg2+ b	H2O2 c
Energy metabolism	Pyruvate metabolism	aceE	pyruvate dehydrogenase, decarboxylase component E1	++		S
(Group A)	Pyruvate metabolism	aceF	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2	++	++
	Pyruvate metabolism	lpd	lipoamide dehydrogenase, E3 component is part of three enzyme complexes	++
	Pyruvate metabolism	lipA	lipoate synthase	++	+
	Propanate metabolism	ackA	acetate kinase A and propionate kinase 2		++	S
	Pentose phosphate pathway	rpe	D-ribulose-5-phosphate 3-epimerase	++		S
	Respiratory chain	cydB	cytochrome d terminal oxidase, subunit II	+	++
	Respiratory chain	yhcB	cytochrome d terminal oxidase, subunit III		++	S
Outer membrane	Lipopolysaccharide biosynthesis	gmhB	D,D-heptose 1,7-bisphosphate phosphatase		+
stabilization	Lipopolysaccharide biosynthesis	lpcA	D-sedoheptulose 7-phosphate isomerase	+	++
(Group B)	Lipopolysaccharide biosynthesis	rfaC	ADP-heptose:LPS heptosyl transferase I		++
	Lipopolysaccharide biosynthesis	rfaD	ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding		++
	Lipopolysaccharide biosynthesis	rfaE	fused heptose 7-phosphate kinase and heptose 1-phosphate adenyltransferase		+
	Lipopolysaccharide biosynthesis	rfaF	ADP-heptose:LPS heptosyltransferase II		+	S
	Lipopolysaccharide biosynthesis	rfaG	glucosyltransferase I		+
	Peptidoglycan-associated lipoprotein	ydcL	predicted lipoprotein	+	+	S
	Peptidoglycan-associated lipoprotein	yfgL	protein assembly complex, lipoprotein component		++	S
	Peptidoglycan-associated lipoprotein	ynbE	predicted lipoprotein	+	+
	Peptidoglycan-associated lipoprotein	nlpI	conserved protein	+	+
	Peptidoglycan-associated lipoprotein	ycdO	conserved protein	+	+
	Outer membrane integrity	pal	peptidoglycan-associated outer membrane lipoprotein		++
	Outer membrane integrity	tolQ	membrane spanning protein in TolA-TolQ-TolR complex		++
	Outer membrane integrity	tolR	membrane spanning protein in TolA-TolQ-TolR complex		++	S
	Outer membrane integrity	yciM	conserved hypothetical protein		+

^aAccording to the data in Figure S4, ratios of growth in the presence of glucose to that in the absence of glucose at 46°C wer estimated. “++” and “+” represent more than 2.0 and 1.5–2.0, respectively.

^bAccording to the data in Figure S4, ratios of growth in the presence of MgCl₂ to that in the absence of MgCl₂ at 46°C were estimated. “++” and “+” represent more than 2.0 and 1.5–2.0, respectively.

^cAccording to the data in Figure S4, ratios of growth in the presence of H₂O₂ to that in the absence of H₂O₂ at 30°C were estimated. “S” represents less than 0.5.