Table 2. Thermotolerant genes identified in this study.

Classification	Sub-classification	Gene	Function	Glca	Mg2+ b	H2O2 c
DNA repair	DNA replication & repair, DSBR	dnaQ	DNA polymerase III subunit, epsilon		+	S
(Group C)	DNA replication & repair, DSBR	holC	DNA polymerase III subunit, chi		++	S
	DNA replication & repair, DSBR	priA	primosome factor n'	++	+	S
	DNA repair, DSBR	ruvA	component of RuvABC resolvasome, endonuclease	++	++	S
	DNA repair, DSBR	ruvC	conserved protein required for cell growth	+	+	S
tRNA modification	tRNA modification	iscS	sulfer relay system, cysteine desulfurase			S
(Group D)	tRNA modification	yheL	sulfer relay system, predicted intracellular sulfur oxidation protein			S
	tRNA modification	yheM	sulfer relay system, predicted intracellular sulfur oxidation protein			S
	tRNA modification	yheN	sulfer relay system, predicted intracellular sulfur oxidation protein			S
	tRNA modification	yhhP	conserved protein required for cell growth			S
	tRNA modification	miaA	delta(2)-isopentenylpyrophosphate tRNA-adenosine transferase		+	S
	tRNA modification	trmU	tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase			S
	tRNA modification	truA	pseudouridylate synthase I	+
Chaperone/protease	Chaperon system	dnaJ	chaperone Hsp40, co-chaperone with DnaK		++	S
(Group E)	Chaperon system	dnaK	chaperone Hsp70, co-chaperone with DnaJ			S
	Chaperon system	degP	chaperone/serine endoprotease
	Chaperon regulator	rseA	anti-sigma factor		++	S
Translation control	Translation control	rpmJ	50S ribosomal subunit L36, related to secY expression			S
(Group F)	Translation control	rpsF	30S ribosomal subunit S6, supecifically modified with glutamic acid or phosphate			S
	Translation control	dksA	DNA-binding transcriptional regulator or rRNA transcription DnaK suppressor	+	+	S
	Translation control	smpB	component of trans-translation process	+	+	S
Cell division	Related to cell division	xerC	site-specific tyrosine recombinse involved in chromosome dimer resolution		++	S
(Group G)	Related to cell division	dedD	membrane-anchored periplasmic protein involved in separation		++
	Related to cell division	envC	regulator of cell wall hydrolases responsible for cell separation		+
Others	Membrane transport	zntA	zinc/cadmium/mercury/lead-exporting ATPase		+	+
	Membrane transport	ybgH	predicted proton-dependent oligopeptide Transporter, POT family	+	++
	Membrane transpor	ybhH	conserved hypothetical protein	++	++	S

^aAccording to the data in Figure S4, ratios of growth in the presence of glucose to that in the absence of glucose at 46°C wer estimated. “++” and “+” represent more than 2.0 and 1.5–2.0, respectively.

^bAccording to the data in Figure S4, ratios of growth in the presence of MgCl₂ to that in the absence of MgCl₂ at 46°C were estimated. “++” and “+” represent more than 2.0 and 1.5–2.0, respectively.

^cAccording to the data in Figure S4, ratios of growth in the presence of H₂O₂ to that in the absence of H₂O₂ at 30°C were estimated. “S” represents less than 0.5.