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. 2011 Jun 10;6(6):e20788. doi: 10.1371/journal.pone.0020788

Figure 7. Clb2 is rate-limiting for mitotic events.

Figure 7

A Clb2 and Pgk1 protein levels for WT (blue) and GALL:CLB2 (red) strains at indicated time points after release from alpha factor. Quantification above shows Clb2 levels normalized to Pgk1 and expressed as a fraction of GALL:CLB2 peak at 60′. B DNA content of the two strains at the indicated timepoints following release, analyzed by FACS. C Budding fraction, polarization index (values around 1 indicate depolarized growth; values >1 indicate polarized growth), metaphase fraction, and anaphase fraction of WT (blue) and GALL-CLB2 (red) cells.