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. Author manuscript; available in PMC: 2011 Jun 13.
Published in final edited form as: Endocrinology. 2010 Apr 6;151(6):2723–2735. doi: 10.1210/en.2010-0007

Figure 6.

Figure 6

Effects of estradiol on nNOS phosphorylation and physical approximation to the plasma membrane in ovariectomized female rats. A. Western blot analyses of the Ser 1412- phosphorylated nNOS isoform (P-nNOS; upper panel) and the non-phosphorylated isoform (nNOS; lower panel) in preoptic region protein extracts from ovariectomized rats treated (OVX+E2) or not (OVX) with estradiol. A representative blot from three independent experiments is shown. B. The protein levels are expressed in arbitrary densitometric units as the ratio between the P-nNOS signal and the signal obtained with nNOS in each sample (* p < 0.05, n = 4 independent experiments per group). C. Preoptic region membrane extracts from OVX and OVX+E2 rats were subjected to electrophoresis and Western-blot. The antibodies used are anti-P-nNOS, anti-nNOS, anti-NR2B and anti-actin. Signal intensities of both P-nNOS (D) and nNOS (E) were quantified and reported in bar graphs (n = 4 independent experiments, ***, p < 0.001). F. Representative bargraph of NOS activity in membrane extracts from preoptic region fragments from OVX (n= 4) or OVX+E2 (n= 4) female rats (***, p < 0.001).