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. 2001 Dec;11(12):2120–2126. doi: 10.1101/gr.205301

Figure 3.

Figure 3

Assessment of the reverse two-hybrid system as a method to further study Caenorhabditis elegans protein interactions. Reverse two-hybrid system. Each yeast diploid expressing one of the 35 conserved interacting protein pairs from C. elegans was tested to identify those that are sensitive to 5-Fluoroorotic acid (5-FOA). Pairs are arranged in the order described in Table 2. Diploids were replica plated onto both SC-Leu-Trp (A) and SC -Leu, -Trp + 0.2% 5-FOA (B). Twenty-seven of thirty-five pairs (77%) were 5-FOA sensitive.