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. 2011 Jun 10;6(6):e20995. doi: 10.1371/journal.pone.0020995

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Genomic organization of the dylt-1 and dylt-2 genes. The positions of X-box promoter motif sequences and the respective deletion mutant alleles are indicated. (B) Northern blot analysis of total RNA from dylt-2(+) and dylt-2(tm2326) mutant worms. In dylt-2(tm2326) mutants, expression of dylt-2 was abolished in comparison with wild-type worms. The blot was also probed for dyf-2 as a control. (C) Functional assays for dylt-1 and dylt-2 mutant worms. Both analyzed deletion mutants demonstrated wild-type responses (+) with regard to fluorescent dye filling (Dyf), avoidance of high osmotic strength (Osm), chemotaxis to sodium chloride (Che) and odorant sensation assays using Diacetyl (Odr). The same results were obtained with dylt-1(ok417);dylt-2(tm2326) double mutants.