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. 2011 Jun 10;6(6):e20793. doi: 10.1371/journal.pone.0020793

Figure 1. EVI1 synergizes with Dnmt3b to repress the regulatory regions of miR-124-3.

Figure 1

A. Diagram of miR-124-3-Luciferase reporter gene. Numbers indicate the nucleotide boundaries of the reporter constructs and they are numbered respect to the stem-loop start in the miR-124-3 gene taken as +1 and indicated by the arrow. B, C. NIH-3T3 cells stably transfected with reporter plasmids (−467/+28 or −340/+28 or −109/+28) were transiently co-transfected with effector plasmids as shown in the panels. Cell extracts were used for quantification of the reporter gene as described in Material and Methods.