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. 2011 Jun 10;6(6):e20793. doi: 10.1371/journal.pone.0020793

Figure 2. EVI1 interacts with de novo DNA methyltransferases.

Figure 2

A. EVI1 (lane 7) but not EVI1-(1+6Mut) (lane 8) interacts with Dnmt3a. 293T cells were co-transfected with Myc-tagged Dnmt3a alone (lanes 2 and 6) or in combination with HA-tagged EVI1 or EVI1-(1+6Mut) (lanes 3 and 7, or lanes 4 and 8, respectively). Two days after transfection cell lysates were collected and incubated with anti-HA beads (lanes 5 to 8) followed by IP. The immunoprecipitated proteins (lanes 5 to 8) and proteins from the cell lysates (lanes 1 to 4) were separated by electrophoresis, transferred to a PVDF membrane and probed as marked in the Figure. Lanes 1 and 5 represent the results with mock transfected cells. B, C. 293T cells were transfected with a plasmid encoding EVI1 (lanes 2 and 5) or EVI1-(1+6Mut) (lanes 3 and 6). Lanes 1 and 4 represent mock-transfected cells. Two days after transfection cell lysates were collected and incubated with anti-Dnmt3a (B) or anti-Dnmt3b (C) antibody (lanes 4 to 6) followed by IP. The immunoprecipitated proteins (lanes 4 to 6) and proteins from cell lysates (lanes 1 to 3) were separated by electrophoresis, transferred to a PVDF membrane and probed as marked in the Figure.