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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2011 Apr 25;10(6):938–948. doi: 10.1158/1535-7163.MCT-10-1120

Figure 1. The side population (SP) is enriched for drug-resistant highly-tumorigenic cells.

Figure 1

(A) FACS plots gated for a Hoechst-negative side population (SP) of J82 bladder cancer and MCF7 breast cancer cells; treatment with Verapamil 50 μM drastically reduced the SPs. (B) Drug resistance of side population (SP), non-side population (NSP) or unfractionated whole population (WP) 24 hours after exposure to cisplatin (25 μM) or docetaxel (0.4 mM for J82, 0.1 mM for MCF7). (C) Colony forming assays comparing NSP to SP and to SP + verapamil (“vera”) + Hoechst33342. Left: Sample images of colony formation; Right: Colonies were quantified by counting those with radius >1mm 3 weeks after plating. (D) Xenograft tumor formation: Left: FACS-fractionated SP or NSP were inoculated subcutaneously into SCID mice at three dilutions (5×104, 1×104 and 1×103) and tumor volumes were measured twice weekly. Right: Wet weights of tumors: For each cell dilution, tumors were excised when the largest tumors exceeded 10mm in greatest diameter. All results are means of independent experiments conducted in triplicate.