Food intake following site-specific microinfusions of E2. Microinfusions of E2 in the MPOA, ARC and DRN, but not in the PVN, LH, or VMH decreased food intake in OVX rats. In the MPOA and ARC (A and B, respectively), the larger dose of E2 decreased 4- and 24-h food intake, whereas the smaller dose of E2 decreased 24-h food intake only. In the DRN (C), dose-related decreases in 24-h food intake were observed following E2 treatment. Neither dose of estradiol affected food intake in the PVN (D), LH (E) or VMH (F). *E2 < vehicle at corresponding time points, P < 0.05. **2.5 μg dose of E2 < 0.25 μg dose of E2, P < 0.05.