Table 1.

Food intake following infusions of vehicle and 2.5 μg estradiol (E2) in rats with misplaced cannulae that failed to target the indicated brain areas.

Brain area	Vehicle	2.5 μg E2	t-test results
MPOA	19.5 ± 0.6 g	20.4 ± 1.0 g	t(4) = 0.702, p = 0.52
ARC	23.1 ± 0.8 g	22.7 ± 0.8 g	t(2*) = 1.245, p = 0.34
DRN	21.9± 1.2 g	21.0 ± 1.6 g	t(6) = 1.073, p = 0.32
PVN	20.9 ± 0.6 g	22.1 ± 2.1 g	t(2) = 0.831, p = 0.49
LH	20.1 ± 2.0 g	22.0 ± 1.7 g	t(2) = 2.495, p = 0.12
VMH	22.9 ± 0.9 g	21.8 ± 0.7 g	t(4) = 0.918, p = 0.41
Combined Nuclei	21.5 ± 0.5 g	21.5 ± 0.6 g	t(25*) = 0.008, p = 0.99

Data are presented as mean ± SEM. Group differences were assessed via dependent t-tests, which are presented in the last column. Twenty-four h food intake was similar following microinfusions of vehicle or E2 in these rats with misplaced cannulae.

^*One rat with a misplaced cannula in the ARC was excluded from the ARC and combined nuclei analyses because the cannula extended into the third ventricle and, as expected, reduced 24-h food intake.

Abbreviations: MPOA = medial preoptic area; ARC = arcuate nucleus; DRN = dorsal raphe nucleus; PVN = paraventricular nucleus; LH = lateral hypothalamus; VMH = ventromedial hypothalamus.