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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Neurochem. 2011 May 19;118(1):140–152. doi: 10.1111/j.1471-4159.2011.07289.x

Fig. 3. L-[3H]glutamine uptake in astrocytes is not modified by hypoosmotic media or by the glutaminase inhibitor DON.

Fig. 3

(a) For measurements of L-glutamine uptake, astrocytes were incubated with L-[3H]glutamine and 2 μM of unlabeled L-glutamine in Basal, −40% Hypo; or Low-Na media for 5–40 minutes. Data are the mean values ±SE of 6 independent measurements. (b) To exclude the contribution of the enzymatic conversion of L-[3H]glutamine to L-[3H]glutamate, astrocytes were treated with or without the glutaminase inhibitor DON (1 mM) for 40 min before transport measurements. L-[3H]glutamine uptake was measured for 40 min in the presence of 2 μM of unlabeled L-glutamine. Results are the means ± SE of 3 experiments.