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. 2011 May 16;11:25. doi: 10.1186/1472-6807-11-25

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Copyright ©2011 Piątek et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The FT-IR results of the DraD-sc denaturiation experiments. (A) Second derivatives of the DraD-sc at temperature range of 29.8 - 73.8°C. Numbers 1 and 2 denote the first and the second feature of two distinguishable transitions. Arrows indicate changes in minima intensities with an increase of temperature. (B) The result of chemometric analysis of denaturation data from the figure A. Three individuals are present (indicated with different colors and symbols), and two transitions are visible near 50°C and 60°C. (C) Second derivatives of the DraD-sc in the presence of 10 mM DTT at temperature range of 29.1 - 70.1°C. Description is the same as in figure A.