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Growth curves of E. coli RV308 pCUlac-pelB-RI (periplasmic expression construct) and E. coli RV308 pCUlac-His6-RI (cytoplasmic production construct) without (control) and with RI production in a batch and a fed-batch process with exponential glucose feeding in a 10 L bioreactor. For conditions see Material and Methods. Black symbols: control culture without induction; red symbols: batch bioreactor cultivation, blue symbols: fed-batch bioreactor cultivation.
