Figure 1.

The LHCGR recombinants and expression in CHO cells. a), The LHCGR cDNA recombinants used for in vitro expression of the receptor protein in CHO cells grown in suspension. The C-terminus of each recombinant contained FLAG tag; b), the cell densities through 7 days of transfection with plasmids R1: LHCGR-318, R2: LHCGR-291 and R3: LHCGR-229 as well as the control (transfection reagents alone). Day 0 is the start of transfection while days 1-7 are post-transfection; c), the cell viability expressed as a percentage of total cell count in plasmid transfected and control cultures through 8 days of the experiment.