Table 2.
Comparisons between two identical plates tested with the Bio-Rad Human 17-plex cytokine panel.
| Cytokine | Number of positive observations | Spearman's Rho | GM (pg/ml) plate 1 | GM (pg/ml) plate 2 |
% difference in GM (95% CI) between 2 plates |
|---|---|---|---|---|---|
| IL-1 β | 31 | 0.88** | 32.47 | 21.34 | 52 (32, 75) |
| IL-2 | 12 | 0.96** | 30.86 | 43.76 | 29 (16, 41) |
| IL-4 | 19 | 0.63** | 1.88 | 1.21 | 55 (35, 78) |
| IL-5 | 7 | 0.71NS | 2.16 | 2.52 | 14 (-23, 40) |
| IL-6 | 19 | 0.95** | 345.36 | 248.76 | 38 (20, 60) |
| IL-10 | 8 | 0.86** | 21.65 | 15.06 | 44 (-19, 155) |
| IL-13 | 18 | 0.86** | 5.30 | 4.49 | 18 (-8, 52) |
| G-CSF | 12 | 0.62* | 118.08 | 51.06 | 131 (92, 179) |
| IFN-γ | 4 | -0.60NS | 85.55 | 103.95 | 18 (-50, 55) |
| MIP-1β | 15 | 0.89** | 156.73 | 59.85 | 162 (117, 217) |
| TNF | 9 | -0.13NS | 61.10 | 40.35 | 51 (2, 126) |
Spearman's Rho was used to test if a significant correlation existed between the samples on the two plates. **= Spearman's Rho test with significance of P < 0.01, * = Spearman's Rho test with significance of P < 0.05, NS = Spearman's Rho test which is non-significant. The cytokines with too few positive observations to enable spearman's Rho tests were not included on this table. GM = geometric mean.