A. Jpx-9 cells were treated with 20 or 40 μM CdCl2 for 1 to 4 days and processed for immunoblot analysis. The membrane was probed with rabbit anti-Tax serum. The Tax band is indicated by an arrow (right). The asterisk indicates a non-specific band.
B. Jpx9 cells were mock treated or treated with 40μM CdCl2 for two days and quantitative RT-PCR was performed from total RNA using Ku70, Ku80 and GAPDH primers. Ku80 and Ku70 values obtained were normalized to their respective GAPDH values. C. Jurkat cells were either mock treated or treated with 40 μM CdCl2 for two days and analyzed by RT PCR as described in panel B.