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. 2010 Dec 5;60(3):173–185. doi: 10.1007/s12013-010-9138-4

Fig. 4.

Fig. 4

Co-localization studies with fluorescently labeled antibodies, as observed by fluorescence microscopy. HeLa cells were treated for 12 h with 5 μM MG-132. Panel A Protein aggregation detected by ProteoStat® dye co-localizes with ubiquitin. a ProteoStat® dye stained, b cells treated with fluorescein-labeled ubiquitin-reactive antibody (clone EX-9) and c composite image. Panel B Protein aggregation detected by ProteoStat® dye co-localizes with fluorescein-labeled p62-reactive antibody. a ProteoStat® dye stained cells, b cells treated with fluorescein-labeled p62-reactive antibody, c composite image. Panel C Protein aggregation detected by ProteoStat® dye co-localizes with LC3. a ProteoStat® dye stained, b cells treated with fluorescein-labeled LC3-reactive antibody and c composite image