Table 2.
Experimental conditions of the screening design and the corresponding responses.
| Run no. |
M/S ratio (ml/gs) |
MgSO4 (mg/gs) |
CaCl2 (mg/gs) |
Moisturizing —agent |
Inoculum size (discs) |
Glucose (mg/gs) |
Ammonium tartrate (mg/gs) |
CuSO4 (mg/gs) |
Measured and estimated laccase activities (U/gds) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4 days |
7 days |
10 days |
13 days |
16 days |
|||||||||
| 1 | 3 | 0.3 | 0.3 | CPB | 4 | 0 | 0 | 0 | 2.16 1.88 |
37.00 38.68 |
2.88 9.93 |
10.00 9.54 |
6.60 10.26 |
| 2 | 3 | 0.3 | 0.3 | SAB | 4 | 30 | 5.5 | 0.75 | 2.74 2.65 |
19.62 20.09 |
1.56 −0.04 |
1.56 1.37 |
0.52 −0.41 |
| 3 | 3 | 0.3 | 0.3 | CPB | 8 | 60 | 11 | 1.5 | 2.74 2.65 |
39.92 40.39 |
13.60 11.99 |
17.04 16.85 |
5.30 4.37 |
| 4 | 3 | 0.3 | 0.3 | SAB | 8 | 0 | 0 | 0 | 3.66 4.12 |
45.16 42.52 |
9.16 5.31 |
3.78 4.62 |
3.46 1.66 |
| 5 | 6 | 0.3 | 3 | CPB | 4 | 30 | 11 | 0 | 3.60 3.92 |
61.52 58.14 |
63.48 63.38 |
74.60 73.93 |
85.08 81.99 |
| 6 | 6 | 0.3 | 3 | SAB | 4 | 0 | 0 | 1.5 | 4.70 5.04 |
63.48 61.83 |
69.38 78.32 |
96.86 93.51 |
108.64 107.09 |
| 7 | 6 | 0.3 | 3 | CPB | 8 | 0 | 0 | 0.75 | 6.60 6.08 |
64.14 66.74 |
103.40 91.25 |
81.16 84.12 |
101.44 101.13 |
| 8 | 6 | 0.3 | 3 | SAB | 8 | 60 | 5.5 | 0 | 5.16 5.02 |
57.60 60.02 |
61.52 64.82 |
67.40 68.45 |
65.44 70.38 |
| 9 | 3 | 3 | 3 | CPB | 4 | 60 | 0 | 0.75 | 1.50 1.88 |
36.00 34.83 |
7.98 12.98 |
2.34 1.70 |
2.02 3.28 |
| 10 | 3 | 3 | 3 | SAB | 4 | 0 | 11 | 0 | 3.26 2.80 |
37.96 42.77 |
15.96 8.91 |
7.46 10.46 |
0.26 4.29 |
| 11 | 3 | 3 | 3 | CPB | 8 | 0 | 5.5 | 0 | 4.50 4.78 |
51.70 47.84 |
15.44 19.28 |
9.42 6.04 |
9.28 3.39 |
| 12 | 3 | 3 | 3 | SAB | 8 | 30 | 0 | 1.5 | 6.40 6.20 |
40.58 40.80 |
1.56 −0.24 |
1.30 2.31 |
2.16 2.76 |
| 13 | 6 | 3 | 0.3 | CPB | 4 | 0 | 5.5 | 1.5 | 4.90 4.85 |
56.94 57.90 |
99.48 93.93 |
87.70 90.22 |
92.28 94.16 |
| 14 | 6 | 3 | 0.3 | SAB | 4 | 60 | 0 | 0 | 3.60 3.45 |
55.62 53.89 |
70.68 63.98 |
61.52 61.30 |
66.76 61.49 |
| 15 | 6 | 3 | 0.3 | CPB | 8 | 30 | 0 | 0 | 6.60 6.57 |
58.24 60.92 |
64.80 68.30 |
57.60 57.44 |
60.20 63.61 |
| 16 | 6 | 3 | 0.3 | SAB | 8 | 0 | 11 | 0.75 | 5.94 6.17 |
63.48 61.57 |
83.12 91.87 |
82.46 80.32 |
80.50 80.48 |