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. 2011 Jun 1;2011:657460. doi: 10.4061/2011/657460

Figure 1.

Figure 1

Growth kinetics of Y-GFP and Y-GFP-ODC clone cultured at different conditions. Cultures carried out under the indicated conditions were diluted to 10–20 × 106 cells/mL when the stationary phase was reached. (a) Y-GFP cultured in rich media BHT (- -⬤- -), Diamond (—■—), and in Diamond supplemented with 2 mg/mL glucose (- -△- -). (b) Y-GFP cultured in the semisynthetic medium SDM79 without (—◆ —) or with (- -◊- -) 1 mM putrescine. (c) Y-GFP-ODC clone cultured in SDM79 (—⬤—) or in SDM79 supplemented with 5 mM α-difluoromethylornithine (DFMO) (- -⬤- -). All cultures were continuously maintained in presence of 250 μg/mL G418.