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. 2011 May 24;2:326. doi: 10.1038/ncomms1327

Figure 5. Analysis of stereoselectivity of the reaction product towards (R,S)-reticuline.

Figure 5

Authentic (R,S)-reticuline (a), the reaction product in the culture of the reticuline-producing strain without NCS (b), the reaction product in the culture of BL21DE3 cells harbouring 6OMT-4′OMT-CNMT/pACYC184 (a plasmid for producing reticuline from norlaudanosoline) (c–f), were analysed by LC–MS. Selected-ion monitoring (SIM) parameter: m/z=330 (reticuline). BL21DE3 cells harbouring 6OMT-4′OMT-CNMT/pACYC184 were grown in LB medium at 25 °C until the OD600 reached 0.5. Then, 1 mM of IPTG and the indicated concentration, 10 μM (c), 30 μM (d), 100 μM (e) and 300 μM (f) of norlaudanosoline were added. Samples were collected at 12 h after induction.