Figure 7. Modelling analyses of PH_PLC-GFP fluorescence intensity induced by stimulation of Ci-VSP phosphatase activity.

A, scheme of the modelling. B, result obtained in the model when diffusion of PH_PLC-GFP was not taken into account. Dotted and continuous lines indicate the experimental data and the modelling results, respectively. The right panel is a magnified view of the decay after the onset of depolarization (arrow). C, result obtained in the model when diffusion of PH_PLC-GFP was taken into account. The experimental data and Ci-VSP density are the same as in B. [Ci-VSP] = 21,894 μm⁻¹, D = 4.5 μm² s⁻¹. Right panel is a magnified view. D, fitting of the PH_PLC-GFP fluorescence data while changing the value of diffusion coefficient (D). The diffusion coefficients were set to 1.0 μm² s⁻¹ for the left panel and 45 μm² s⁻¹ for the right panel. Dotted and continuous lines indicate the experimental data shown in B and the results estimated from the model, respectively. The colour code is the same as in B.