. 2011 Apr 4;589(Pt 11):2687–2705. doi: 10.1113/jphysiol.2011.208165

Table 2.

Equations for the modelling

Reaction	Rate equation
Dephosphorylation of PI(4,5)P₂ by PI-5 phosphatase	k_PI5Phos×[PI(4,5)P₂]
Phosphorylation of PI(4)P by PI-5 kinase	k_PI5K×[PI(4)P]
Binding of PI(4,5)P₂ to PH_PLC-GFP	k_f,PHD×[PI(4,5)P₂]× PHD_GFP_cyto−k_r,PHD× PHD_GFP_PI(4,5)P₂
Ci-VSP activity	V_max×[PI(4,5)P₂]/ (K_M+[PI(4,5)P₂])
Reaction rate between n_o and n_c	k_f,Kir×n_c×[PI(4,5)P₂]–k_r,Kir×n_o

k_PI5Phos: rate constant of the reaction to dephosphorylate PI(4,5)P₂; [PI(4,5)P₂]: density of PI(4,5)P₂ on plasma membrane; [PI(4)P]: density of PI(4)P on plasma membrane; k_PI5K: rate constant of the reaction to phosphorylate PI(4)P; k_f,PHD: rate constant of the reaction that PI(4,5)P₂ binds to PH_PLC-GFP; k_r,PHD: rate constant of the reaction that PI(4,5)P₂ dissociates from the complex of PH_PLC-GFP and PI(4,5)P₂; PHD_GFP_cyto: concentration of PH_PLC-GFP in cytosol; PHD_GFP-PI(4,5)P₂: density of PH_PLC-GFP bound to PI(4,5)P₂ on plasma membrane; k_f,Kir and k_r,Kir: rate constant of the reaction that Kir2.1 channel binds to and dissociates from the PI(4,5)P₂, respectively; n_c and n_o: densities of closed and open Kir channel, respectively.