Figure 1.

The distribution of transgenic fyn mRNA in the brain. (A) Transgenic fyn mRNA was detected in horizontal brain sections prepared from F₁ offspring of fyn transgenic mice using a digoxigenin-labeled transgene-specific cRNA probe. No signal was detected in nontransgenic littermates (non-Tg). The regional pattern restricting to the forebrain was essentially similar among the individual lines. (B) In the forebrain of line N8, the transgenic fyn mRNA was expressed highly in neurons of the neocortex (Cx), hippocampal pyramidal layer (Py), dentate granular layer (DG), and lateral amygdala nucleus (LA). Scale bar, 500 μm. (C) In the cerebellum of line N8, the transgenic fyn mRNA was also detected in Purkinje cells (Pu). (ML) Cerebellar molecular layer; (GL) cerebellar granular layer; (WM) white matter. Scale bar, 100 μm.