Figure 4.

Recognition of a TGFβ1-like polypeptide on Western blots. (A) A blot, probed with the anti-TGFβ-1 antibody, showing the prominent 28-kD polypeptide (arrow) in an extract of hemocytes, and authentic recombinant vertebrate TGFβ1 (arrowhead). (B) Blot of hemocytes and a detergent extract of gauze that had been implanted in an animal for 4 days. The anti-TGFβ1 antibody detected a prominent 28-kD polypeptide in the gauze extract (arrow). Much of the antigen in the hemocyte sample was found in high molecular weight polypeptides.